How do I set up my LightBench?
After you receive your LightBench, installation and performance verification should be performed before using it to process samples. The Installation Checklist ships with the instrument, and along with the provided USB key should guide you through the setup steps. These include ensuring an appropriate location is chosen, installing software, and ensuring the instrument is working. This instructional video covers the unpacking and setup process, and this instructional video shows the instrument qualification process.
How do I set up a run in the software?
Setting up a run in the software involves a number of steps to define both the samples being run and the cassette being used. Start by selecting “File → New → Run” and then select an appropriate deck map file which matches the cassette you are using. The next steps are defined on the right hand side panel starting with “Create Run”. This instructional video shows the steps of setting up a size selection run.
How do I integrate Ranger Technology into my LIMS?
Ranger Technology is designed to be easily integrated into any LIMS. All input required to start an electrophoresis or mass quantitation process can be specified in .csv files, and all data generated is output in machine readable .csv or .xml file formats. To find this data, look for the “Output” folder in the run directory. For more information, see section 5.6.7 of the LightBench User Manual.
How do I integrate a LightBench with another robot?
Integrating the LightBench with other instruments, for example to perform the liquid handling during a run can be accomplished with several different tools. You can use the LightBench SiLA (Version 2.0) server which allows for a centralized client to discover and command the LightBench in concert with any other SiLA compatible devices. Another solution for automation is to use the RobotInterface program which uses a .NET API to control the instrument.
How do I choose what type of cassette and loading buffer to use?
When selecting the appropriate reagents, important factors include the expected base pair range of your sample, as well as the total mass, volume and salt content. In order to achieve the necessary resolution, the gel percentage must match the length of DNA fragments being loaded. The graphic below shows the same ladder with bands ranging from 50bp to 20,000bp, and how they resolve in our four different gel percentages.
What should I do if my LightBench experiences an error?
Errors related to analysis of the electrophoresis images can be addressed directly through the UI. Section 5.4.4 of the LightBench User Manual provides step-by-step instructions for this.
If an instrument-level error interrupts electrophoresis, the run can typically be resumed without compromising the samples or the results.
For further support call +1 (604) 620 1332 or email firstname.lastname@example.org.
How do I order reagents?
To order reagents to go https://www.coastalgenomics.com/order-reagents/ and specify the details of the kit you would like to order including cassette type, gel percentage, and Loading Buffer markers. If you need help selecting the right reagents for your application contact email@example.com.
How do I upgrade the software or firmware on my LightBench?
Updates to the software and firmware of the LightBench can be accessed from http://coastalgenomics.com/software. This page contains a chart which details the latest firmware and software updates, as well as documentation of the changes in each version. Note that newer versions of firmware have a “minimum required software version” to function, while newer versions of software are backward compatible with older versions of firmware.
To install software, download the installer and follow the instructions provided. Refer to the release notes to determine if an “Upgrade” or a “Full Installation” is required.
To install the firmware, start by downloading the .HEX file. Then launch the Configuration Utility (Start->Programs->Coastal Genomics->Launcher->”Configuration”) and connect to the LightBench by selecting the COM port from the drop down menu and click “Connect”. Then click the “Open Interface” button in the Firmware Programming section. Click “Program Firmware” and then select the .HEX file. Programming the firmware can take a few minutes, after which the new firmware version will be displayed in the “Post Programming” field.
How can I view the data generated during my electrophoresis run?
You can view analytics data by either launching the Analytics application (RangerAnalytics.exe) from the SizeSelection application (SSR.exe) by navigating to the “Tools → Analytics” menu item. If you have a run loaded in the SSR software which has output analytics data, that data will be loaded automatically using this method. Otherwise, you can load the analytics data manually from within the Analytics application by clicking load and then selecting the MidRunElectropherogram (either .xml or .csv) located at:
C:\Program Files (x86)\Coastal Genomics\SSR\Runs\[TIMESTAMP]_[RUN NAME]\Output
This instructional video shows how to interact with the Analytics application, including how to generate a PDF report.
What regular maintenance is required for the LightBench?
After each usage, clean up any spilled buffer from the drawer with a KimWipe.
If the LightBench is moved to a different location, we recommend performing a camera calibration routine, by launching the software and navigating to the “Instrument → Service → Calibrate Camera” menu item and then following the instructions shown.
We recommend performing a Preventative Maintenance (PM) procedure every 6 months. To request a quote for a service technician to perform a PM procedure, or for more information about what the procedure entails contact firstname.lastname@example.org.